anti raf1 (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc anti raf1
Anti Raf1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 643 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/raf1/c-Raf+Antibody/pm41664072-84-28-30
Average 96 stars, based on 643 article reviews

anti raf1 - by Bioz Stars, 2026-10

96/100 stars

Images

Western Blot:

Article Title: High Levels of miR-7-5p Potentiate Crizotinib-Induced Cytokilling and Autophagic Flux by Targeting RAF1 in NPM-ALK Positive Lymphoma Cells
Article Snippet: The protein lysates were fractionated on SDS-PAGE (10% or 15%), and transferred to a nitrocellulose membrane (Whatman) (GE Healthcare, Little Chalfont, UK). .. Western blotting was performed using ALK (D5F3 XP, Cell Signaling Technology #3633, Ozyme, Saint Cyr l’Ecole, France), phospho-ALK (Y1507) (Cell Signaling Technology, #14678), LC3-B (Cell Signaling Technology #2775), p62 (BD Transduction Laboratories #610832), ULK1 (Cell Signaling Technology #6439), phospho-ULK1 (Ser757) (Cell Signaling Technology #14202), RAF1 (Cell Signaling Technology #3738), MEK1/2 (Cell Signaling Technology #9122), phospho-MEK1/2 (Ser217/221) (Cell Signaling Technology #9121), ERK1/2 (Cell Signaling Technology #9102), phospho-ERK1/2 (Thr202/Tyr204) (Cell Signaling Technology #9106), β-actin (Santa Cruz #7210, Heidelberg, Germany), and HSP90 (Origene #TA332385, Rockville, MD, USA) antibodies. .. The proteins were visualized using the ClarityTM ECL Western Blotting Substrate (Bio-Rad, Hercules, CA, USA).

Article Title: Loss of mitochondrial Ca 2+ response and CaMKII/ERK activation by LRRK2 R1441G mutation correlate with impaired depolarization-induced mitophagy
Article Snippet: The quantification of band intensity was done using the Image Lab Software (Bio-Rad). α-Tubulin served as the loading control for all immunoblots, as its molecular weight (in kilodaltons) is not close to proteins of interest. .. Immunoblotting was done using the following primary antibodies: Phospho-p44/42 MAPK (MAPK/ERK1/2) (Thr202/Tyr204) (1:5000; Cell Signaling TechnologyTM, 4376 S), p44/42 MAPK (MAPK/ERK1/2) (1:5000; Cell Signaling TechnologyTM, 4695 S), phospho-MAP2K/MEK1/2 (Ser217/221) (1:2000; Cell Signaling TechnologyTM, 9121 S), MAP2K/MEK1/2 (1:2000; Cell Signaling TechnologyTM, 9122 S), Phospho-RAF1 (Ser338) (1:1000; Cell Signaling TechnologyTM, 9427 S), RAF1 (1:1000; Cell Signaling TechnologyTM, 9422 S), phospho-Thr73 Rab10 (1:2000; AbcamTM, ab230261), Rab10 (1:1000; Cell Signaling TechnologyTM, 8127 S), Phospho-Drp1 (Ser616) (1:1000; Cell Signaling TechnologyTM, 3455 S), Drp1 (1:1000; Cell Signaling TechnologyTM, 8570), phospho-CaMKII (Thr287) (1:2000; ThermoFisherTM Scientific, PA5-37833), CaMKII (1:2000; AbcamTM, ab52476), Phospho-CaMKI (Thr177) (1:3000; ThermoFisherTM Scientific, PA5-37833), CaMKI (1:3000; AbcamTM, ab68234), MCU (1:3000; AbcamTM, ab219827), COX4 (1:2000; AbcamTM, ab16056), NCLX (1:1000; AbcamTM, ab83551) and α-Tubulin (1:5000; Cell Signaling TechnologyTM, 9099 S). ..

other:

Article Title: YWHAE-NUTM2 oncoprotein regulates proliferation and cyclin D1 via RAF/MAPK and Hippo pathways
Article Snippet: Polyclonal antibodies to p-RB1 (S807/811, #9308), p-RAF1 (S259, #9421), p-BRAF (S445, #2696), p-MEK (S217/221, #9121), p-MAPK (T202/Y204, #9101), MEK (#9122), MAPK (#9102), and YAP/TAZ (#8418) were from Cell Signaling Technology (Beverly, MA), whereas those for RAF1 (sc-7267), CTGF (sc-14939) and CYR61 (sc-13100) were from Santa Cruz Biotechnology (Santa Cruz, CA).

Membrane:

Article Title: RAF1 amplification drives a subset of bladder tumors and confers sensitivity to MAPK-directed therapeutics
Article Snippet: .. Sections of the membrane corresponding to the desired protein molecular weights were stained overnight with primary antibodies: RAF1 (1:1000; catalog 9422, CST), BRAF (1:1000; clone D9T6S, CST), ERK1/2 (1:1000; clone 3A7, CST), AKT (1:1000; clone C67E7, CST), phospho-MEK1/2 (1:1000; clone 41G9, CST), phospho-ERK1/2 (1:1000; clone D13.14.4E, CST), phospho-AKT(1:1000; clone D9E, CST), total and cleaved PARP (1:1000, catalog 9542, CST), phospho-H2AX Ser139 (1:1000, clone JBW301, MilliporeSigma), or β-tubulin (1:1000, clone G-8, Santa Cruz Biotechnology) prepared in 1% milk in TBST. .. A LI-COR Odyssey Infrared Imaging System was used for signal detection using IRDYE-conjugated secondary antibodies (LI-COR Biosciences).

Article Title: High Levels of miR-7-5p Potentiate Crizotinib-Induced Cytokilling and Autophagic Flux by Targeting RAF1 in NPM-ALK Positive Lymphoma Cells
Article Snippet: .. The kinase reaction was stopped by the addition of Laemmli buffer 2×, and the samples were then heated for 5 min at 95 °C, loaded onto SDS-PAGE, transferred to a nitrocellulose membrane, and probed with the RAF1 (Cell Signaling Technology #3738), total ULK1 (Cell Signaling Technology #6439), and p-ULK1 (Ser 757) (Cell Signaling Technology #14202) antibodies. ..

Staining:

Planar Chromatography:

Article Title: HIIT-induced lactate/GPR81 signaling with dual branches converging on ERK1/2 contributes to hippocampal synaptic remodeling and memory improvement
Article Snippet: After a 15-min blocking step (PS108P, Epizyme, Shanghai, China), the samples were incubated with primary antibodies overnight at 4 °C. .. GPR81, PA5-114741, Thermo; AC (55067-1-AP, Proteintech), PKA (4782S, CST), RAP1 (2399S, CST), PKC (SC-80 Santa), PLC (14247S, CST), Raf1 (9422S, CST), MSK1 (3489S, CST), P90RSK (9355S, CST), ERK (4370T, CST), CREB (9197S, CST), c-Fos (2250S, CST), Zif268 (4154S, CST), NMDAR (5704S, CST), SYN (4329S, CST), BDNF (28205-1-AP, Proteintech), ARC (16290-1-AP, Proteintech), PSD95 (3450S, CST) p-Raf1 (9421S, CST), p-ERK (4370T, CST), p-MSK (9595S, CST), p-P90RSK (11989S, CST), p-CREB (9198S, CST), Tubulin (10068-1-AP, Proteintech), HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H + L) (SA00001-2, Proteintech), HRP-conjugated Affinipure Goat Anti-Mouse IgG (H + L) (SA00001-1, Proteintech). ..

Review